Garlic extracts were obtained and dilution susceptibility assay was used to determine. Garlic extracts reported activities include reducing. The antibacterial activity of the crude extract of garlic was investigated against clinical and standard isolates of s. Studies on the antimicrobial effects of garlic allium sativum linn, ginger zingiber officinale roscoe and lime citrus aurantifolia linn. Comparatively, garlic extracts inhibited bacterial growths than tetracycline and penicillin.
Some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in gastrointestinal infections. Antibacterial effect of garlic allium sativum staphyloccus. Pdf antimicrobial properties of allium sativum garlic. Various garlic preparations have been shown to exhibit a wide spectrum of antibacterial activity against gramnegative and grampositive bacteria including species of escherichia, salmonella, staphylococcus, streptococcus, klebsiella, proteus, bacillus, and clostridium. The antibacterial activity associated with garlic allium sativum l. Antimicrobial activity garlic s infectionfighting capability was confirmed in a study conducted by researchers at the university of ottawa that was published in the april 2005 issue of phytotherapy research. Six metal ions led, iron, copper, zinc, calcium and magnesium 1% were used. Antibacterial activity of garlic extract against human pathogenic. Comparison of antimicrobial activity of allium sativum cloves. Antibacterial activity of syzygium aromaticum clove with.
Effect of metal ions on antibacterial properties of methanolic extract. Thiosulfinates play an important role in the antibiotic activity of garlic. Similarly, in eight males aged 40e50 years, the consumption of one crushed clove of garlic daily for 16 weeks resulted in an 80% reduction in serum thromboxane b 2. Antibacterial activity of allium sativum against staphylococcus aureus in vitro 347 was 27% at a wave length of 580 nanometer, while the light transmittance was 100% for nutrient broth used to prepare the bacteria jassim, 2003. Resurgence in the use of natural herbal alternatives has brought. The observation of the study also indicate that the inhibitory effect of allicin active principle of garlic on gram positive bacteria. Antimicrobial activity of ginger zingiber officinale. The antimicrobial activities of garlic and other plant alliums are primarily based on allicin, a thiosulphinate present in crushed garlic bulbs. In addition, larger clear zones were observed at higher concentrations against both microorganisms. Apr 27, 2012 the antibacterial activity was determined by disc diffusion method. Antibacterial activity of garlic extract on streptomycinresistant staphylococcus aureus and escherichia coli solely and in synergism with streptomycin. The antibacterial activity of garlic is widely attributed to allicin. Antimicrobial activity was tested by using 3 different processes chopping, freezing, and slicing by the disk diffusion method. Grampositive staphylococcus aureus atcc baa 1026 and gramnegative escherichia coli atcc 10536 were made resistant to standard antibiotic streptomycin used as a control.
In addition, garlic was shown to have antimicrobial activity. Diallylthiosulfinate allicin, a volatile antimicrobial from garlic. Try the experiment again, only this time, use garlic powder. Comparative study of antibacterial activity of garlic and cinnamon at different temperature and its application on preservation of fish shivendu ranjan, nandita dasgupta, proud saha, madhumita rakshit and c. Comparatively, 50 and 100 mll concentrations of onions extracts were less inhibitory than 200, 300 and 500 mll concentrations. The antibacterial effect of ginger and garlic extracts on. Agar diffusion method the molten agar will mixed with a different concentrations of the test samples at molten state 4550c and mixed aseptically with different amounts of garlic. The minimum inhibitory concentration mic of different bacterial species varied from 0. A comparison of the antibacterial and antifungal activities. The results showed that tr garlic presented more antimicrobial activity than cn garlic. Currently, interest has been focused on exploring antimicrobial properties of plants and herbs.
Antibacterial activity of the crude extracts of garlic was evaluated against. Garlic oil demonstrated significant antibacterial activity, particularly against methicillinresistant staphylococcus. Extracts of garlic rich in allicin and ajoene showed appreciable antimycobacterial activity as compared to standard drugs. Antimicrobial activity of essential oil extracts of various. Pdf on apr 5, 2014, atheer khashan published antibacterial activity of garlic extract allium sativum against staphylococcus aureus in vitro. The study focused on the garlic antibacterial activity on s.
The purpose of this study is to determine quantitative antimicrobial activity of garlic against shigella by detecting the mic. It reacts very rapidly with free thiol groups, via thioldisulphide. Antibacterial activity of garlic, tulsi, bitter guard and cinnamon extracts against wound pathogens godfrey omare mauti, eliakim mbaka mauti, geoffrey arasa ouno, benard maronga mabeya in the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter. Garlic contains a higher concentration of sulfur compounds which are responsible for its medicinal effects. The test isolates showed variable susceptibility to the garlic and ginger extract aqueous and ethanolic and to other antibiotics cloxacillin, cefepime, cefoxitin, clindamycin and tobramycin. Garlic extract has antimicrobial activity against many genera of bacteria, fungi and viruses. Also,upon reduction of allicin to diallyl disulfide,the antibacterial activity is greatly reduced. Antimycobacterial and antibacterial activity of allium. Apr 30, 2018 allicin diallylthiosulfinate is a defence molecule from garlic allium sativum l. This project work will be limited only to anti microbial activity of garlic and ginger extracts against staphylococcus aureus. Antibacterial activity of garlic extract allium sativum against staphylococcus aureus in vitro atheer abdulhameed khashan college of pharmacy, anbar university, iraq abstract the study was conducted to investigate the inhibitory effect of alcoholic extract of garlic on the inhibition of growth of. All tested bacterial strains were most susceptible to the garlic aqueous extract and showed poor susceptibility to the ginger aqueous extract. Hughes and lawson showed that the antimicrobial activity of garlic is completely abolished when the thiosulfinates e.
The healing properties of garlic are wide and varied, ranging from antioxidant, antifungal, antiviral and antibacterial properties to cancerfighting and immuneboosting activity. Previous authors have described the antibacterial activity of garlic extract against microorganisms. It is formed rapidly by the action of the enzyme, allinase. Allicin in its pure form was found to exhibit i antibacterial activity against a wide range of gramnegative and grampositive bacteria, including multidrugresistant enterotoxicogenic strains of escherichia coli. Antimicrobial properties of allicin from garlic sciencedirect. The antibacterial activity of garlic oil was evaluated relative to staphylococcus aureus, escherichia coli and bacillus subtilis in the inhibition zone by the use of amoxicillin as a standard preparation elmahmood, 2009. The antibacterial effect of garlic allium safivum and other.
It is known that allicin has sulfhydryl modifying activity and is capable of inhibiting sulfhydryl enzymes. Antibacterial effect of garlic allium sativum journal of infectious. Allicin, one of the active principles of freshly crushed garlic homogenates, has a variety of antimicrobial activities. Infection benefits from garlic garlic howstuffworks. H2 garlic and ginger extracts mixed show enhanced anti microbial activity against staphylococcus aureus. Inhibitory effect of allium sativum and zingiber officinale.
Preparaton of standard dilutions of garlic extract the dilution were prepared by using ethylene glycol which. Due to its healing properties, raw garlic has been used as a medicinal plant to prevent and in some cases treat or even heal various health complaints. H1 ginger and garlic extracts do not show anti microbial activity on staphylococcus aureus. We set out to determine if pure allicin and aqueous garlic extracts age exhibit antimicrobial properties against the burkholderia cepacia complex bcc, the major bacterial phytopathogen for alliums and an intrinsically multiresistant and life. Ramalingam school of bioscience and technology, vit university, vellore, tamil nadu, india. This study focuses the significant antibacterial activity of garlic allium sativum linn. Antibacterial activity of plant extract against three food associated bacteria were examined in the presence of metal ions. Antibacterial activity of garlic extract on cariogenic. Antibacterial effect of garlic allium sativum against. Cooking garlic, however, destroys the allicin, so youll need to use raw garlic to prevent or fight infections.
Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by two variants of the agar diffusion. The antibacterial activity test of the crude extract of garlic against both standard and clinical isolates were carried out by the agardiffusion method. Antibacterial and antimicrobial properties science. Garlic, a botanical that grows in iowa, was compared with a standard antibacterial nursery dietary regimen. Bulbs belonging to the allium genus had the most antibacterial activity against streptococcus mutans ohara et al. Pdf antibacterial activity of garlic, tulsi, bitter guard. While some of the human medicines work against very specific microorganisms, garlic is antibacterial, antifungal, antiparasitic, antiprotozoan and antiviral. The antibacterial activity of the crude extracts of garlic was evaluated against standard isolates of s. While some workers 18, 19 observed that the antimicrobial activity of clove on e. In vitro antibacterial activity and stability of garlic. The essential oil eo extracts of these allium plants garlic and onions exhibited marked antibacterial activity, with garlic showing the highest inhibition and green onion the lowest. Four different solvents having different polarity were used to extract the bioactive compound from garlic.
Allicin and its derivatives inhibit the cysteine protease, thereby acting antiparasitic on the human and animal. Effect of vermicompost on yield, quality, and antibacterial. Antimicrobial properties of garlic oil against human enteric bacteria. In the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter guard and cinnamon, were explored against staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa, which had been. Pdf antimicrobial activity of garlic extract against. The antibacterial properties of crushed garlic have been known for a long time. Pdf antibacterial activity of garlic extract allium sativum against. The antibacterial activity of extracts and oil was estimated by zone of inhibition method. Antibacterial activity of garlic extract on streptomycinresistant.
Allium sativum journal of chemical and pharmaceutical research. Pdf garlic allium sativum l is a species of monocot, bulbforming perennial scented herb belonging to family amaryllidacea. Antibacterial activity of garlic extract on streptomycin. Grampositive staphylococcus aureus atcc baa 1026 and gramnegative escherichia coli atcc 10536 were made resistant to standard antibiotic streptomycin used as a control in the experiment. Mechanism of activity of cn garlic could be proposed to be different from that of tr garlic. Comparative study of antibacterial activity of garlic and. Although garlic has been used for its medicinal properties for thousands of years, investigations into its mode of action are relatively recent.
Garlic extracts are known to inhibit the growth of fungi, protozoa, viruses, and numerous bacteria, namely salmonella spp. Pdf on apr 5, 2014, atheer khashan published antibacterial activity of garlic extract allium sativum against staphylococcus aureus in vitro find, read and cite all the research you need on. Pdf antibacterial activity of garlic extract allium. Efficacy of antimicrobial activity of aqueous garlic allium sativum extract against different bacterial species. Antibacterial activity of boenninghausenia albiflora reichb. Antibacterial activity of garlic, tulsi, bitter guard and.